OPTIMAL FEULGEN HYDROLYSIS FOR MICROSPECTROPHOTOMETRY
نویسندگان
چکیده
منابع مشابه
Microspectrophotometry of Cell Nuclei Stained with the Feulgen Reaction
1. DNA contents of the individual parenchymal nuclei of rat livers during postnatal growth were estimated by microspectrophotometric apparatus, and different ploidy classes of nuclei were classified by their DNA contents. With the same material the total number of parenchymal nuclei in the liver was counted microscopically. 2. If the DNA content of nuclei encountered most frequently in several ...
متن کاملCritical evaluation of Feulgen microspectrophotometry for estimating amount of DNA in cell nuclei.
In recent years, microspectrophotometry of the Feulgen stain (1) has been extensively used as a method for determining amounts of desoxyribosenucleic acid (DNA) in individual cell nuclei (2-4). In spite of certain obvious advantages of this procedure which permits the DNA analysis of a single nucleus in situ directly under the microscope and its applicability to the study of the significance of...
متن کاملFeulgen Hydrolysis of Normal Cells and Mouse Ascites Tumor Cells
The effect of HCl hydrolysis on the dye content (Feulgen reaction) of normal cells and mouse ascites tumor cells was examined by means of cytophotometric measurements. After 11 min of hydrolysis, 16-day-old tumor cells showed a hypotetraploid DNA line with doubling peaks. The DNA values were in the ratios of 1:2:4:8 during all the tested hydrolysis times (3 to 21 min). The size of the nucleus a...
متن کاملA Chromatographic Study of Hydrolysis in the Feulgen Nucleal Reaction
In a study on Feulgen hydrolysis of frozen-dried alcohol-fixed lily anthers, a chromatographic technique was developed to analyze the acid hydrolysate for some of the degradation products of nucleic acid. Hydrolysis was accomplished by 10 per cent perchloric acid at 20 degrees C., and a typical hydrolysis time-Feulgen intensity curve was obtained, with maximum staining occurring at 19 hours. Mi...
متن کاملA Chromatographic Study of Hydrolysis in the Feulgen Nucleal Reaction* by Philip
Acid hydrolysis of tissue has long been recognized as a necessary part of the Feulgen method for demonstrating the presence of DNA (deoxyribonucleic acid) in histological preparations (Feulgen and Rossenbeck, 1924). When attempts were made to use the method for the quantitative determination of DNA (Pollister and Ris, 1947; Di Stefano, 1948; Ris and Mirsky, 1949; Swift, 1950 a), the role of hyd...
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ژورنال
عنوان ژورنال: ACTA HISTOCHEMICA ET CYTOCHEMICA
سال: 1971
ISSN: 0044-5991,1347-5800
DOI: 10.1267/ahc.4.166